01669nas a2200121 4500008004100000245017100041210006900212520113400281100002001415700002501435700003101460856005601491 2007 eng d00aA shift toward harmonics for high-frequency calling shown with phylogenetic study of frequency spectra in Eneopterinae crickets (Orthoptera, Grylloidea, Eneopteridae)0 ashift toward harmonics for highfrequency calling shown with phyl3 a

Dominant frequency is an important feature of cricket song, as it can be used for species recognition and mate choice. The dominant frequency usually ranges from 2 to 8 kHz and corresponds to the fundamental frequency of the song. In preliminary studies we documented high-frequency songs in the subfamily Eneopterinae. To analyse this phenom- enon further, we investigate the spectral properties of these cricket songs. Based on the analysis of the frequency spectra in reference to phylogeny, we show that a shift of dominant frequency from the fundamental toward the second or the third harmonic of the song occurred in the Lebinthini tribe. In this clade the upper harmonics (second or third) become the most powerful frequencies of the song. From an evolutionary point of view, we document a case of convergence toward high-frequency calling between species of Eneoptera Burmeister, 1838, which emit alternatively low and high fundamental frequencies, and the Lebinthini, which emit high frequencies using upper harmonics. Functional hypotheses are discussed to explain how these high frequencies could be produced.

1 aROBILLARD, TONY1 aGrandcolas, Philippe1 aDesutter-Grandcolas, Laure uhttp://www.nrcresearchpress.com/doi/10.1139/Z07-10602624nas a2200181 4500008004100000245015900041210006900200520194700269100001902216700002002235700003102255700002002286700002502306700001702331700002002348700002102368856005302389 2016 eng d00aOld Lineage on an Old Island: Pixibinthus, a New Cricket Genus Endemic to New Caledonia Shed Light on Gryllid Diversification in a Hotspot of Biodiversity0 aOld Lineage on an Old Island Pixibinthus a New Cricket Genus End3 a

Few studies have focused on the early colonization of New Caledonia by insects, after the re-emergence of the main island, 37 Myr ago. Here we investigate the mode and tempo of evolution of a new endemic cricket genus, Pixibinthus, recently discovered in southern New Caledonia. First we formally describe this new monotypic genus found exclusively in the open shrubby vegetation on metalliferous soils, named ‘maquis minier’, unique to New Caledonia. We then reconstruct a dated molecular phylogeny based on five mitochondrial and four nuclear loci in order to establish relationships of Pixibinthus within Eneopterinae crickets. Pixibinthus is recovered as thesister clade of the endemic genus Agnotecous, mostly rainforest-dwellers. Dating results show that the island colonization by their common ancestor occurred around 34.7 Myr, shortly after New Caledonia re-emergence. Pixibinthus and Agnotecous are then one of the oldest insect lineages documented so far for New Caledonia. This discovery highlights for the first time two clear-cut ecological specializations between sister clades, as Agnotecous is mainly found in rainforests with 19 species, whereas Pixibinthus is found in open habitats with a single documented species. The preference of Pixibinthus for open habitats and of Agnotecous for forest habitats nicely fits an acoustic specialization, either explained by differences in body size or in acoustic properties of their respective habitats. We hypothesize that landscape dynamics, linked to major past climatic events and recent change in fire regimes are possible causes for both present-day low diversity and rarity in genus Pixibinthus. The unique evolutionary history of this old New Caledonian lineage stresses the importance to increase our knowledge on the faunal biodiversity of ‘maquis minier’, in order to better understand the origin and past dynamics of New Caledonian biota.

1 aAnso, Jérémy1 aBarrabé, Laure1 aDesutter-Grandcolas, Laure1 aJourdan, Hervé1 aGrandcolas, Philippe1 aDong, Jiajia1 aROBILLARD, TONY1 aFontaneto, Diego uhttps://dx.plos.org/10.1371/journal.pone.015092003010nas a2200181 4500008004100000245009100041210006900132520234200201653002902543653001202572653002402584653004202608653003302650653002302683100002202706700001902728856008102747 2013 eng d00aDiversity of acoustic tracheal system and its role for directional hearing in crickets0 aDiversity of acoustic tracheal system and its role for direction3 a

Background: Sound localization in small insects can be a challenging task due to physical constraints in deriving sufficiently large interaural intensity differences (IIDs) between both ears. In crickets, sound source localization is achieved by a complex type of pressure difference receiver consisting of four potential sound inputs. Sound acts on the external side of two tympana but additionally reaches the internal tympanal surface via two external sound entrances. Conduction of internal sound is realized by the anatomical arrangement of connecting trachea. A key structure is a trachea coupling both ears which is characterized by an enlarged part in its midline (i.e., the acoustic vesicle) accompanied with a thin membrane (septum). This facilitates directional sensitivity despite an unfavorable relationship between wavelength of sound and body size. Here we studied the morphological differences of the acoustic tracheal system in 40 cricket species (Gryllidae, Mogoplistidae) and species of outgroup taxa (Gryllotalpidae, Rhaphidophoridae, Gryllacrididae) of the suborder Ensifera comprising hearing and non hearing species.

Results: We found a surprisingly high variation of acoustic tracheal systems and almost all investigated species using intraspecific acoustic communication were characterized by an acoustic vesicle associated with a medial septum. The relative size of the acoustic vesicle - a structure most crucial for deriving high IIDs - implies an important role for sound localization. Most remarkable in this respect was the size difference of the acoustic vesicle between species; those with a more unfavorable ratio of body size to sound wavelength tend to exhibit a larger acoustic vesicle. On the other hand, secondary loss of acoustic signaling was nearly exclusively associated with the absence of both acoustic vesicle and septum.

Conclusion: The high diversity of acoustic tracheal morphology observed between species might reflect different steps in the evolution of the pressure difference receiver; with a precursor structure already present in ancestral non-hearing species. In addition, morphological transitions of the acoustic vesicle suggest a possible adaptive role for the generation of binaural directional cues.

10aAcoustic tracheal system10acricket10adirectional hearing10aInteraural intensity difference (IID)10aPressure difference receiver10aSound localization1 aSchmidt, Arne, KD1 aRömer, Heiner uhttp://frontiersinzoology.biomedcentral.com/articles/10.1186/1742-9994-10-6101645nas a2200253 4500008004100000022001400041245018200055210006900237260001600306300000600322490000900328520081100337653001901148653001701167653001401184653001601198653001301214653001701227100002001244700002501264700001901289700002601308856005701334 2014 eng d a1175-532600aRevision of the cricket genus Cardiodactylus (Orthoptera, Eneopterinae, Lebinthini): the species from both sides of the Wallace line, with description of 25 new species0 aRevision of the cricket genus emCardiodactylusem Orthoptera Eneo cAug-08-2015 a10 v38543 a

The genus Cardiodactylus is the most speciose and widely distributed genus of the cricket subfamily Eneopterinae and of the Lebinthini tribe. Along with diverse acoustic features, this genus is also characterized by a wide distribution area running from Japan to Southeast Asia, Northern Australia and in many archipelagos in the Western Pacific, with a high contrast in species distributions. In this paper we start revising Cardiodactylus by focusing on the western region of its wide distribution and the Novaeguineae species group. We describe 25 new species of Cardiodactylus, redescribe 3 species and bring new signalizations for 5 species. Whenever possible, information is provided about species distribution, male calling song and male and female genitalia, forewing venation and habitat.

10aCardiodactylus10aEneopterinae10aGryllidae10anew species10arevision10aWallace line1 aROBILLARD, TONY1 aGOROCHOV, ANDREJ, V.1 aPOULAIN, SIMON1 aSUHARDJONO, YAYUK, R. uhttp://biotaxa.org/Zootaxa/issue/view/zootaxa.3854.100454nas a2200145 4500008004100000022001300041245005600054210005300110260001600163300001400179490000700193100001900200700002300219856006600242 2016 eng d a0960982200aSignal Evolution: 'Shaky' Evidence for Sensory Bias0 aSignal Evolution Shaky Evidence for Sensory Bias cJan-01-2016 aR78 - R800 v261 aPascoal, Sonia1 aBailey, Nathan, W. uhttp://linkinghub.elsevier.com/retrieve/pii/S096098221501439602397nas a2200181 4500008004100000022001300041245008400054210006900138260001600207300001600223490000700239520181000246100002902056700002302085700002002108700002102128856006602149 2015 eng d a0960982200aEvolution of a Communication System by Sensory Exploitation of Startle Behavior0 aEvolution of a Communication System by Sensory Exploitation of S cJan-12-2015 a3245 - 32520 v253 a

New communication signals can evolve by sensory exploitation if signaling taps into preexisting sensory biases in receivers [1 ;  2]. For mate attraction, signals are typically similar to attractive environmental cues like food [3; 4; 5 ;  6], which amplifies their attractiveness to mates, as opposed to aversive stimuli like predator cues. Female field crickets approach the low-frequency calling song of males, whereas they avoid high-frequency sounds like predatory bat calls [7]. In one group of crickets (Eneopterinae: Lebinthini), however, males produce exceptionally high-frequency calling songs in the range of bat calls [8], a surprising signal in the context of mate attraction. We found that female lebinthines, instead of approaching singing males, produce vibrational responses after male calls, and males track the source of vibrations to find females. We also demonstrate that field cricket species closely related to the Lebinthini show an acoustic startle response to high-frequency sounds that generates substrate vibrations similar to those produced by female lebinthine crickets. Therefore, the startle response is the most likely evolutionary origin of the female lebinthine vibrational signal. In field crickets, the brain receives activity from two auditory interneurons; AN1 tuned to male calling song controls positive phonotaxis, and AN2 tuned to high-frequency bat calls triggers negative phonotaxis [9 ;  10]. In lebinthine crickets, however, we found that auditory ascending neurons are only tuned to high-frequency sounds, and their tuning matches the thresholds for female vibrational signals. Our results demonstrate how sensory exploitation of anti-predator behavior can evolve into a communication system that benefits both senders and receivers.

1 aHofstede, Hannah, M. ter1 aSchöneich, Stefan1 aRobillard, Tony1 aHedwig, Berthold uhttp://linkinghub.elsevier.com/retrieve/pii/S096098221501358501719nas a2200157 4500008004100000022001300041245013500054210006900189260001600258300001400274490000700288520114900295100002001444700003101464856006601495 2006 eng d a1055790300aPhylogeny of the cricket subfamily Eneopterinae (Orthoptera, Grylloidea, Eneopteridae) based on four molecular loci and morphology0 aPhylogeny of the cricket subfamily Eneopterinae Orthoptera Gryll cJan-09-2006 a643 - 6610 v403 a

The phylogenetic relationships of 39 species of Eneopterinae crickets are reconstructed using four molecular markers (16S rRNA, 12S rRNA, cytochrome b , 18S rRNA) and a large morphological data set. Phylogenetic analysis via direct optimisation of DNA sequence data using parsimony as optimality criterion is done for six combinations of weighting parameter sets in a sensitivity analysis . The results are discussed in a twofold purpose: W rst, in term of signi W cance of the molecular markers for phylogeny reconstruction in Ensifera, as our study represents the W rst molecular phylogeny performed for this insect suborder at this level of diversity; second, in term of corrobora- tion of a previous phylogeny of Eneopterinae, built on morphological data alone. The four molecular markers all convey phylogen etic signal, although variously distributed on the tree. The monophyly of the subfamily, that of three over W ve tribes, and of 10 over 13 genera, are recovered. Finally, previous hypotheses on the evolution of acoustic devices and signals in the Eneopterinae clade are brie X y tested, and supported, by our new data set.

1 aRobillard, Tony1 aDesutter-Grandcolas, Laure uhttp://linkinghub.elsevier.com/retrieve/pii/S105579030600130801171nas a2200157 4500008004100000022001400041245012200055210006900177260001600246300001400262490000700276520059100283100003100874700002000905856008800925 2004 eng d a1399-560X00aA revision of Neotropical Eneopterinae crickets (Orthoptera, Grylloidea, Eneopteridae) with a phylogenetic discussion0 arevision of Neotropical Eneopterinae crickets Orthoptera Grylloi cJan-10-2004 a411 - 4350 v353 a

A revision of Eneopterinae crickets from the Neotropical region is achieved in a cladistic perspective using for the first time characters of male and female genitalia and male forewing venation. Each genus is redefined and its monophyly assessed with reference to all known Eneopterinae genera. 6 new species are described (Eneoptera gracilis, E. nigripedis, Ligypterus belmontensis, L. linharensis, L. pernambucensis and Ponca hebardi) and an identification key is proposed for both males and females. Phylogenetic relationships are discussed and distributions characterised.

1 aDesutter-Grandcolas, Laure1 aRobillard, Tony uhttp://booksandjournals.brillonline.com/content/journals/10.1163/18763120478891242701779nas a2200241 4500008004100000245013100041210006900172300001200241490000900253520099200262653001401254653002201268653001501290653001501305653004201320100001701362700002601379700003401405700001801439700001901457700002001476856004101496 2017 eng d00aComplete mitochondrial genome and taxonomic revision of Cardiodactylus muiri Otte, 2007 (Gryllidae: Eneopterinae: Lebinthini)0 aComplete mitochondrial genome and taxonomic revision of Cardioda a101-1160 v42683 a

In the present study, we report the high-coverage complete mitochondrial genome (mitogenome) of the cricket Cardio- dactylus muiri Otte, 2007. The mitogenome was sequenced using a long-PCR approach on an Ion Torrent Personal Ge- nome Machine (PGM) for next generation sequencing technology. The total length of the amplified mitogenome is 16,328 bp, representing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one noncoding region (D-loop region). The new sets of long-PCR primers reported here are invaluable resources for future comparative evolu- tionary genomic studies in Orthopteran insects. The new mitogenome sequence is compared with published cricket mito- genomes. In the taxonomic part, we present new records for the species and describe life-history traits, habitat and male calling song of the species; based on observation of new material, the species Cardiodactylus buru Gorochov & Robillard, 2014 is synonymized under C. muiri.

10aIndonesia10along-PCR approach10amitogenome10aNew Guinea10anext generation sequencing technology1 aDong, Jiajia1 aVicente, Natallia, M.1 aChintauan-Marquier, Ioana, C.1 aRamadi, Cahyo1 aDettai, Agnès1 aRobillard, Tony uhttps://bio.acousti.ca/id/node/4764801033nas a2200217 4500008004100000022001400041245012700055210006900182260001600251300001400267490000700281520035100288653001300639653001800652653001900670653001500689653001600704100002600720700002000746856004900766 2017 eng d a1280-955100aLigypterus najtae n. sp. from Mounts Tumuc-Humac in French Guiana (Orthoptera, Grylloidea, Gryllidae, Eneopterinae)0 aiLigypterus najtaei n sp from Mounts TumucHumac in French Guiana cJan-03-2017 a125 - 1360 v393 a

Ligypterus najtae n. sp., a new species of Neotropical Eneopterinae Saussure, 1874 cricket, is described from Mounts Tumuc-Humac in French Guiana. Description focusses on general morphology, male and female genitalia, and forewing venation. Bioacoustical analyses of the calling song reveal that this species uses high-frequency signals.

10acrickets10aFrench Guiana10ahigh frequency10aLebinthini10anew species1 aVicente, Natallia, M.1 aRobillard, Tony uhttp://www.bioone.org/doi/10.5252/z2017n1a14