02700nas a2200229 4500008004100000022001400041245013400055210006900189260002000258300001400278490000700292520196200299653002302261653001702284653001502301653001502316100001902331700001602350700001502366700002002381856006902401 2016 eng d a0046-225X00aThe Complete Mitochondrial Genome and Song Evolution of the Monotypic Genus U. Tarbinsky, 1932 (Orthoptera: Tettigoniidae)0 aComplete Mitochondrial Genome and Song Evolution of the Monotypi cOct-19-20162019 a737 - 7460 v453 a

The insect Uvarovites inflatus Uvarov is highly appreciated in China. It is known for its distinctive songs and horn-like forewings and is raised commercially for insect lovers. U. inflatus was previously categorized as part of the mono- typic genus Uvarovites; however, there was little molecular evidence to support this taxonomic classification. This study obtained and investigated the mitogenome of U. inflatus, and its songs were characterized and compared with other Ensifera species whose mitogenomes are available. By performing the mitochondrial analysis, we were able to assess the phylogenetic relationships between these species and discuss the evolution of Ensifera calling songs. The mitogenome of U. inflatus is 15,956bp in length and contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The organization and orientation of the U. inflatus mitogenome are similar to those of other Tettigonioidea species. Phylogenetic analysis based on 13 protein-coding genes showed that the superfamily Tettigonioidea is monophyletic, as are the other six tested subfamilies from Tettigonioidea. Our results also indicated that Grylloidea is monophyletic. A Bayesian relaxed clock analysis showed that the differentiation of U. inflatus and Gampsocleis gratiosa Brunner occurred in the middle Miocene, suggesting that their speciation occurred over a long evolutionary period. The results provide significant support for the establishment of the monotypic genus Uvarovites. Calling song analysis showed that at least two discrete steps of independent evolution occurred during the change from pure tone to broadband noise, and that the ancestor of existing Ensifera was more likely to have emitted pure-tone songs than broadband signals. Together, the mitogenome, molecular clock, and acoustic data allowed us to clarify the taxonomic state of U. inflatus and propose a timeline for the evolution of Ensifera songs.

10aadaptive evolution10ainsect songs10amitogenome10aOrthoptera1 aWang, Yinliang1 aZhao, Hanbo1 aZhang, Xue1 aRen, Bing-Zhong uhttps://academic.oup.com/ee/article-lookup/doi/10.1093/ee/nvw03101779nas a2200241 4500008004100000245013100041210006900172300001200241490000900253520099200262653001401254653002201268653001501290653001501305653004201320100001701362700002601379700003401405700001801439700001901457700002001476856004101496 2017 eng d00aComplete mitochondrial genome and taxonomic revision of Cardiodactylus muiri Otte, 2007 (Gryllidae: Eneopterinae: Lebinthini)0 aComplete mitochondrial genome and taxonomic revision of Cardioda a101-1160 v42683 a

In the present study, we report the high-coverage complete mitochondrial genome (mitogenome) of the cricket Cardio- dactylus muiri Otte, 2007. The mitogenome was sequenced using a long-PCR approach on an Ion Torrent Personal Ge- nome Machine (PGM) for next generation sequencing technology. The total length of the amplified mitogenome is 16,328 bp, representing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one noncoding region (D-loop region). The new sets of long-PCR primers reported here are invaluable resources for future comparative evolu- tionary genomic studies in Orthopteran insects. The new mitogenome sequence is compared with published cricket mito- genomes. In the taxonomic part, we present new records for the species and describe life-history traits, habitat and male calling song of the species; based on observation of new material, the species Cardiodactylus buru Gorochov & Robillard, 2014 is synonymized under C. muiri.

10aIndonesia10along-PCR approach10amitogenome10aNew Guinea10anext generation sequencing technology1 aDong, Jiajia1 aVicente, Natallia, M.1 aChintauan-Marquier, Ioana, C.1 aRamadi, Cahyo1 aDettai, Agnès1 aRobillard, Tony uhttps://bio.acousti.ca/nl/node/47648